https://repositorio.ufba.br/handle/ri/13114
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | São Pedro, André | - |
dc.contributor.author | Detoni, Cássia Britto | - |
dc.contributor.author | Ferreira, Domingos | - |
dc.contributor.author | Albuquerque, Elaine Christine de Magalhães Cabral | - |
dc.contributor.author | Sarmento, Bruno | - |
dc.creator | São Pedro, André | - |
dc.creator | Detoni, Cássia Britto | - |
dc.creator | Ferreira, Domingos | - |
dc.creator | Albuquerque, Elaine Christine de Magalhães Cabral | - |
dc.creator | Sarmento, Bruno | - |
dc.date.accessioned | 2013-10-03T20:36:12Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 0269-3879 | - |
dc.identifier.uri | http://www.repositorio.ufba.br/ri/handle/ri/13114 | - |
dc.description | Texto completo: acesso restrito. p.966–972 | pt_BR |
dc.description.abstract | A reversed-phase high performance liquid chromatography method has been developed and validated for determination and quantitation of the natural sesquiterpene (−)-α-bisabolol. Furthermore the application of the method was done by characterization of chitosan milispheres and liposomes entrapping Zanthoxylum tingoassuiba essential oil, which contains appreciable amount of (−)-α-bisabolol. A reversed-phase C18 column and gradient elution was used with the mobile phase composed of (A) acetonitrile–water–phosphoric acid (19:80:1) and (B) acetonitrile. The eluent was pumped at a flow rate of 0.8 mL/min with UV detection at 200 nm. In the range 0.02–0.64 mg/mL the assay showed good linearity (R2 = 0.9999) and specificity for successful identification and quantitation of (−)-α-bisabolol in the essential oil without interfering peaks. The method also showed good reproducibility, demonstrating inter-day and intra-day precision based on relative standard deviation values (up to 3.03%), accuracy (mean recovery of 100.69% ± 1.05%) and low values of detection and quantitation limits (0.0005 and 0.0016 mg/mL, respectively). The method was also robust for showing a recovery of 98.81% under a change of solvent in standard solutions. The suitability of the method was demonstrated by the successful determination of association efficiency of the (−)-α-bisabolol in chitosan milispheres and liposomes. | pt_BR |
dc.language.iso | en | pt_BR |
dc.source | http://dx.doi.org/10.1002/bmc.1208 | pt_BR |
dc.subject | Chitosan | pt_BR |
dc.subject | Liposomes | pt_BR |
dc.subject | (−)-α-bisabolol | pt_BR |
dc.subject | Method validation | pt_BR |
dc.subject | RP-HPLC | pt_BR |
dc.title | Validation of a high-performance liquid chromatography method for the determination of (-)-α-bisabolol from particulate systems | pt_BR |
dc.title.alternative | Biomedical Chromatography | pt_BR |
dc.type | Artigo de Periódico | pt_BR |
dc.identifier.number | v. 23, n. 9 | pt_BR |
dc.embargo.liftdate | 10000-01-01 | - |
Aparece nas coleções: | Artigo Publicado em Periódico (FAR) |
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