Campo DC | Valor | Idioma |
dc.contributor.author | Keyburn, Anthony L. | - |
dc.contributor.author | Portela, Ricardo Wagner Dias | - |
dc.contributor.author | Sproat, Kathy | - |
dc.contributor.author | Ford, Mark E. | - |
dc.contributor.author | Bannam, Trudi L. | - |
dc.contributor.author | Yan, Xuxia | - |
dc.contributor.author | Rood, Julian I. | - |
dc.contributor.author | Moore, Robert J. | - |
dc.creator | Keyburn, Anthony L. | - |
dc.creator | Portela, Ricardo Wagner Dias | - |
dc.creator | Sproat, Kathy | - |
dc.creator | Ford, Mark E. | - |
dc.creator | Bannam, Trudi L. | - |
dc.creator | Yan, Xuxia | - |
dc.creator | Rood, Julian I. | - |
dc.creator | Moore, Robert J. | - |
dc.date.accessioned | 2013-11-18T21:08:52Z | - |
dc.date.available | 2013-11-18T21:08:52Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 0928-4249 | - |
dc.identifier.uri | http://repositorio.ufba.br/ri/handle/ri/13716 | - |
dc.description | p. 1-8 | pt_BR |
dc.description.abstract | NetB toxin from
Clostridium perfringens
is a major virulence factor in necrotic enteritis in poultry. In this study the
efficacy of NetB as a vaccine antigen to protect chickens from necrotic enteritis was examined. Broiler chickens
were immunized subcutaneously with purified recombinant NetB (rNetB), formalin treated bacterin and cell free
toxoid with or without rNetB supplementation. Intestinal lesion scores and NetB antibody levels were measured to
determine protection after mild oral gavage, moderate in-feed and heavy in-feed challenges with virulent
C.
perfringens
isolates. Birds immunized with rNetB were significantly protected against necrotic enteritis when
challenged with a mild oral dose of virulent bacteria, but were not protected when a more robust challenge was
used. Bacterin and cell free toxoid without rNetB supplementation did not protect birds from moderate and severe
in-feed challenge. Only birds immunized with bacterin and cell free toxoid supplemented with rNetB showed
significant protection against moderate and severe in-feed challenge, with the later giving the greatest protection.
Higher NetB antibody titres were observed in birds immunized with rNetB compared to those vaccinated with
bacterin or toxoid, suggesting that the in vitro levels of NetB produced by virulent
C. perfringens
isolates are too
low to induce the development of a strong immune response. These results suggest that vaccination with NetB
alone may not be sufficient to protect birds from necrotic enteritis in the field, but that in combination with other
cellular or cell-free antigens it can significantly protect chickens from disease. | pt_BR |
dc.language.iso | en | pt_BR |
dc.publisher | Veterinary Research | pt_BR |
dc.source | http://www.veterinaryresearch.org/content/pdf/1297-9716-44-54.pdf | pt_BR |
dc.title | Vaccination with recombinant NetB toxin partially protects broiler chickens from necrotic enteritis | pt_BR |
dc.title.alternative | Veterinary Research | pt_BR |
dc.type | Artigo de Periódico | pt_BR |
dc.description.localpub | Salvador | pt_BR |
Aparece nas coleções: | Artigo Publicado em Periódico (EMV)
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