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Use este identificador para citar ou linkar para este item: https://repositorio.ufba.br/handle/ri/14314
Tipo: Artigo de Periódico
Título: Enhanced Leishmania braziliensis infection following pre-exposure to sandfly saliva
Título(s) alternativo(s): PLoS Neglected Tropical Diseases
Autor(es): Moura, Tatiana Rodrigues de
Oliveira, Fabiano
Novais, Fernanda O.
Miranda, José Carlos
Clarêncio, Jorge
Follador, Ivonise
Carvalho Filho, Edgar Marcelino de
Valenzuela, Jesus G.
Barral-Netto, Manoel
Barral, Aldina Maria Prado
Brodskyn, Claudia Ida
Oliveira, Camila Indiani de
Autor(es): Moura, Tatiana Rodrigues de
Oliveira, Fabiano
Novais, Fernanda O.
Miranda, José Carlos
Clarêncio, Jorge
Follador, Ivonise
Carvalho Filho, Edgar Marcelino de
Valenzuela, Jesus G.
Barral-Netto, Manoel
Barral, Aldina Maria Prado
Brodskyn, Claudia Ida
Oliveira, Camila Indiani de
Abstract: Background: Sand fly saliva has an array of pharmacological and immunomodulatory components, and immunity to saliva protects against Leishmania infection. In the present study, we have studied the immune response against Lutzomyia intermedia saliva, the main vector of Leishmania braziliensis in Brazil, and the effects of saliva pre-exposure on L. braziliensis infection employing an intradermal experimental model. Methodology/principal findings: BALB/c mice immunized with L. intermedia salivary gland sonicate (SGS) developed a saliva-specific antibody response and a cellular immune response with presence of both IFN-γ and IL-4. The inflammatory infiltrate observed in SGS-immunized mice was comprised of numerous polymorphonuclear and few mononuclear cells. Mice challenged with live L. braziliensis in the presence of saliva were not protected although lesion development was delayed. The inoculation site and draining lymph node showed continuous parasite replication and low IFN-γ to IL-4 ratio, indicating that pre-exposure to L. intermedia saliva leads to modulation of the immune response. Furthermore, in an endemic area of cutaneous leishmaniasis, patients with active lesions displayed higher levels of anti-L. intermedia saliva antibodies when compared to individuals with a positive skin test result for Leishmania. Conclusion: These results show that pre-exposure to sand fly saliva plays an important role in the outcome of cutaneous leishmaniasis, in both mice and humans. They emphasize possible hurdles in the development of vaccines based on sand fly saliva and the need to identify and select the individual salivary candidates instead of using whole salivary mixture that may favor a non-protective response.
Palavras-chave: Leishmania braziliensis
Saliva
Leishmania - Immunology
País: Brasil
Tipo de Acesso: Acesso Aberto
URI: http://repositorio.ufba.br/ri/handle/ri/14314
Data do documento: 2007
Aparece nas coleções:Artigo Publicado em Periódico (Faculdade de Medicina)

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