Campo DC | Valor | Idioma |
dc.contributor.author | Melo, Stella Maria Barrouin | - |
dc.contributor.author | Poester, Fernando Padilla | - |
dc.contributor.author | Ribeiro, Marcos Borges | - |
dc.contributor.author | Alcântara, Adriano Costa de | - |
dc.contributor.author | Aguiar, Paulo Henrique Palis | - |
dc.contributor.author | Nascimento, Ivana Lúcia de Oliveira | - |
dc.contributor.author | Schaer, Robert Eduard | - |
dc.contributor.author | Nascimento, Roberto José Meyer | - |
dc.contributor.author | Freire, Songelí Menezes | - |
dc.creator | Melo, Stella Maria Barrouin | - |
dc.creator | Poester, Fernando Padilla | - |
dc.creator | Ribeiro, Marcos Borges | - |
dc.creator | Alcântara, Adriano Costa de | - |
dc.creator | Aguiar, Paulo Henrique Palis | - |
dc.creator | Nascimento, Ivana Lúcia de Oliveira | - |
dc.creator | Schaer, Robert Eduard | - |
dc.creator | Nascimento, Roberto José Meyer | - |
dc.creator | Freire, Songelí Menezes | - |
dc.date.accessioned | 2014-01-21T21:14:43Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 0034-5288 | - |
dc.identifier.uri | http://repositorio.ufba.br/ri/handle/ri/14421 | - |
dc.description | Texto completo: acesso restrito. p. 340-346 | pt_BR |
dc.description.abstract | An indirect ELISA test was developed for the diagnosis of Brucella canis infection in dogs. A bacterial whole cell extract was used as a solid phase antigen, using B. canis isolated from an infected animal. Sera from culture-positive and healthy negative animals were used as internal reference controls. The cut-off point was determined by a mathematical formula for a statistically valid value, which defined the upper prediction limit, based on the upper tail of the t-distribution of 21 negative control sera readings, for the confidence level of 99.5%. The sensitivity and specificity of the ELISA test were 95% and 91%, respectively. The ELISA test showed a significant concordance index (K = 0.84) with the agar gel immunodiffusion test. The reliability of the ELISA for the detection of infected animals was established by a double blind study testing 280 sera provided by serum banks from different diagnostic and research institutions and analyzed by ROC Curve. | pt_BR |
dc.language.iso | en | pt_BR |
dc.rights | Acesso Aberto | pt_BR |
dc.source | http://dx.doi.org/10.1016/j.rvsc.2007.02.006 | pt_BR |
dc.subject | Brucella canis | pt_BR |
dc.subject | ELISA | pt_BR |
dc.subject | Serodiagnosis | pt_BR |
dc.subject | Canine brucellosis | pt_BR |
dc.title | Diagnosis of canine brucellosis by ELISA using an antigen obtained from wild Brucella canis | pt_BR |
dc.title.alternative | Research in Veterinary Science | pt_BR |
dc.type | Artigo de Periódico | pt_BR |
dc.identifier.number | v. 83, n. 3 | pt_BR |
dc.embargo.liftdate | 10000-01-01 | - |
Aparece nas coleções: | Artigo Publicado em Periódico (EMV)
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