https://repositorio.ufba.br/handle/ri/14493
Tipo: | Artigo de Periódico |
Título: | Simultaneous Determination of Caffeine, Theobromine, and Theophylline by High-Performance Liquid Chromatography |
Título(s) alternativo(s): | Journal of Chromatographic Science |
Autor(es): | Bispo, Márcia Sousa Oliveira, Rodolfo F. S. de Reis, José Oscar N. Andrade, Jailson Bittencourt de Veloso, Márcia Cristina da Cunha Pinheiro, Heloísa Lúcia C. |
Autor(es): | Bispo, Márcia Sousa Oliveira, Rodolfo F. S. de Reis, José Oscar N. Andrade, Jailson Bittencourt de Veloso, Márcia Cristina da Cunha Pinheiro, Heloísa Lúcia C. |
Abstract: | This work relates the development of an analytical methodology to simultaneously determine three methylxanthines (caffeine, theobromine, and theophylline) in beverages and urine samples based on reversed-phase high-performance liquid chromatography. Separation is made with a Bondesil C18 column using methanol-water-acetic acid or ethanol-water-acetic acid (20:75:5, v/v/v) as the mobile phase at 0.7 mL/min. Identification is made by absorbance detection at 273 nm. Under optimized conditions, the detection limit of the HPLC method is 0.1 pg/mL for all three methylxanthines. This method is applied to urine and to 25 different beverage samples, which included coffee, tea, chocolate, and coconut water. The concentration ranges determined in the beverages and urine are: < 0.1 pg/mL to 350 µg/mL and 3.21 µg/mL to 71.2 µg/mL for caffeine; < 0.1pg/mL to 32 µg mL and < 0.1 pg/mL to 13.2 µg/mL for theobromine; < 0.1 pg/mL to 47 µg/mL and < 0.1 pg/mL to 66.3 µg/mL for theophylline. The method proposed in this study is rapid and suitable for the simultaneous quantitation of methylxanthines in beverages and human urine samples and requires no extraction step or derivatization. |
Tipo de Acesso: | Acesso Aberto |
URI: | http://repositorio.ufba.br/ri/handle/ri/14493 |
Data do documento: | 2002 |
Aparece nas coleções: | Artigo Publicado em Periódico (Química) |
Arquivo | Descrição | Tamanho | Formato | |
---|---|---|---|---|
Marcia S. Bispo.pdf | 145,43 kB | Adobe PDF | Visualizar/Abrir |
Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.