Campo DC | Valor | Idioma |
dc.contributor.author | Souza, Cleide dos Santos | - |
dc.contributor.author | Chicaybam, Leonardo | - |
dc.contributor.author | Bonamino, Martin Hernán | - |
dc.contributor.author | Bahia, Marcus Vinicius | - |
dc.contributor.author | Costa, Silvia Lima | - |
dc.contributor.author | Borges, Helena L. | - |
dc.contributor.author | Rehen, Stevens K. | - |
dc.creator | Souza, Cleide dos Santos | - |
dc.creator | Chicaybam, Leonardo | - |
dc.creator | Bonamino, Martin Hernán | - |
dc.creator | Bahia, Marcus Vinicius | - |
dc.creator | Costa, Silvia Lima | - |
dc.creator | Borges, Helena L. | - |
dc.creator | Rehen, Stevens K. | - |
dc.date.accessioned | 2014-03-13T18:24:48Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 1547-3287 | - |
dc.identifier.uri | http://repositorio.ufba.br/ri/handle/ri/14750 | - |
dc.description | Texto completo: acesso restrito. p. 1711-1720 | pt_BR |
dc.description.abstract | Flavonoids have key functions in the regulation of multiple cellular processes; however, their effects have been poorly examined in pluripotent stem cells. Here, we tested the hypothesis that neurogenesis induced by all-trans retinoic acid (RA) is enhanced by agathisflavone (FAB, Caesalpinia pyramidalis Tull). Mouse embryonic stem (mES) cells and induced pluripotent stem (miPS) cells growing as embryoid bodies (EBs) for 4 days were treated with FAB (60 μM) and/or RA (2 μM) for additional 4 days. FAB did not interfere with the EB mitotic rate of mES cells, as evidenced by similar percentages of mitotic figures labeled by phospho-histone H3 in control (3.4%±0.4%) and FAB-treated groups (3.5%±1.1%). Nevertheless, the biflavonoid reduced cell death in both control and RA-treated EBs from mES cells by almost 2-fold compared with untreated EBs. FAB was unable, by itself, to induce neuronal differentiation in EBs after 4 days of treatment. On the other hand, FAB enhanced neuronal differentiation induced by RA in both EBs of mES and miPS. FAB increased the percentage of nestin-labeled cells by 2.7-fold (mES) and 2.4 (miPS) and β-tubulin III–positive cells by 2-fold (mES) and 2.7 (miPS) in comparison to RA-treated EBs only. FAB increased the expression of RA receptors α and β in mES EBs, suggesting that the availability of RA receptors is limiting RA-induced neurogenesis in pluripotent stem cells. This is the first report to describe that naturally occurring biflavonoids regulate apoptosis and neuronal differentiation in pluripotent stem cells. | pt_BR |
dc.language.iso | en | pt_BR |
dc.rights | Acesso Aberto | pt_BR |
dc.source | http://dx.doi.org/ 10.1089/scd.2010.0446 | pt_BR |
dc.title | Agathisflavone Enhances Retinoic Acid-Induced Neurogenesis and Its Receptors α and β in Pluripotent Stem Cells | pt_BR |
dc.title.alternative | Stem Cells and Development | pt_BR |
dc.type | Artigo de Periódico | pt_BR |
dc.identifier.number | v. 20, n. 10 | pt_BR |
dc.embargo.liftdate | 10000-01-01 | - |
Aparece nas coleções: | Artigo Publicado em Periódico (ICS)
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