Campo DC | Valor | Idioma |
dc.contributor.author | Bueris, Vanessa | - |
dc.contributor.author | Sircili, Marcelo Palma | - |
dc.contributor.author | Taddei, Carla Romano | - |
dc.contributor.author | Santos, Maurilio Fernandes dos | - |
dc.contributor.author | Franzolin, Marcia Regina | - |
dc.contributor.author | Martinez, Marina Baquerizo | - |
dc.contributor.author | Ferrer, Suzana Ramos | - |
dc.contributor.author | Barreto, Mauricio Lima | - |
dc.contributor.author | Trabulsi, Luiz Rachid | - |
dc.creator | Bueris, Vanessa | - |
dc.creator | Sircili, Marcelo Palma | - |
dc.creator | Taddei, Carla Romano | - |
dc.creator | Santos, Maurilio Fernandes dos | - |
dc.creator | Franzolin, Marcia Regina | - |
dc.creator | Martinez, Marina Baquerizo | - |
dc.creator | Ferrer, Suzana Ramos | - |
dc.creator | Barreto, Mauricio Lima | - |
dc.creator | Trabulsi, Luiz Rachid | - |
dc.date.accessioned | 2011-06-18T04:10:13Z | - |
dc.date.available | 2011-06-18T04:10:13Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 1678-8060 | - |
dc.identifier.uri | http://www.repositorio.ufba.br/ri/handle/ri/1551 | - |
dc.description | p. 839-844 | pt_BR |
dc.description.abstract | We identified different diarrheagenic (DEC) Escherichia coli pathotypes isolated from 1,207 children with and without acute endemic diarrhea in Salvador, Bahia, Brazil collected as part of a case control study. Since the identification of DEC cannot be based on only biochemical and culture criteria, we used a multiplex polymerase chain reaction developed by combining five specific primer pairs for Enteropathogenic Escherichia coli (EPEC), Shiga toxin-producing E. coli/ Enterohaemorrhagic E. coli (STEC/EHEC), Enterotoxigenic E. coli (ETEC) and Enteroaggregative E. coli (EAEC) to detect these pathotypes simultaneously in a single-step reaction. In order to distinguish typical and atypical EPEC strains, these were tested for the presence of EAF plasmid. The prevalence of diarrheagenic E. coli in this sample of a global case-control study was 25.4% (259 patients) and 18.7% (35 patients) in the diarrhea group (1,020 patients) and the control group (187 patients), respectively. The most frequently isolated pathotype was EAEC (10.7%), followed by atypical EPEC (9.4%), ETEC (3.7%), and STEC (0.6%). Typical EPEC was detected only in one sample. The prevalence of the pathotypes studied in children with diarrhea was not significantly different from that in children without diarrhea. | pt_BR |
dc.language.iso | en | pt_BR |
dc.subject | diarrheagenic Escherichia coli | pt_BR |
dc.subject | virulence | pt_BR |
dc.subject | Multiplex PCR | pt_BR |
dc.title | Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazil | pt_BR |
dc.title.alternative | Mem Inst Oswaldo Cruz | pt_BR |
dc.type | Artigo de Periódico | pt_BR |
dc.description.localpub | Rio de Janeiro | pt_BR |
dc.identifier.number | 102(7) | pt_BR |
Aparece nas coleções: | Artigo Publicado em Periódico Nacional (ISC)
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