https://repositorio.ufba.br/handle/ri/20780
Tipo: | Artigo de Periódico |
Título: | Role of IFN-gamma and LPS on neuron/glial co-cultures infected by Neospora caninum |
Autor(es): | Jesus, Erica Etelvina Viana de Santos, Alex Barbosa dos Ribeiro, Catia Suse Oliveira Pinheiro, Alexandre Moraes Freire, Songeli Menezes El-Bachá, Ramon dos Santos Costa, Silvia Lima Costa, Maria de Fatima Dias |
Autor(es): | Jesus, Erica Etelvina Viana de Santos, Alex Barbosa dos Ribeiro, Catia Suse Oliveira Pinheiro, Alexandre Moraes Freire, Songeli Menezes El-Bachá, Ramon dos Santos Costa, Silvia Lima Costa, Maria de Fatima Dias |
Abstract: | Neospora caninum causes cattle abortion and neurological symptoms in dogs. Although infection is usually asymptomatic, classical neurological symptoms of neosporosis may be associated with encephalitis. This parasite can grow in brain endothelial cells without markedly damages, but it can modulate the cellular environment to promote its survival in the brain. In previous studies, we described that IFN-g decreased the parasite proliferation and down regulated nitric oxide (NO) production in astrocyte/microglia cultures. However,it remains unclear how glial cells respond to N. caninum in the presence of neurons. Therefore, we evaluated the effect of 300 IU/mL IFN-gamma or 1.0 mg/mL of LPS on infected rat neuron/glial co-cultures. After 72 h of infection, LPS did not affect the mitochondrial dehydrogenase activity. However, IFN-gamma decreased this parameter by 15.5 and 12.0% in uninfected and infected cells, respectively. The number of tachyzoites decreased 54.1 and 44.3% in cells stimulated with IFN-gamma and LPS, respectively. Infection or LPS treatment did not change NO production. On the other hand, IFN-gamma induced increased nitrite release in 55.7%, but the infection reverted this induction. IL-10 levels increased only in infected cultures (treated or not), meanwhile PGE2 release was improved in IFN-gamma/infected or LPS/infected cells. Although IFN-gamma significantly reduced the neurite length in uninfected cultures (42.64%; p < 0.001), this inflammatory cytokine reverted the impairment of neurite outgrowth induced by the infection (81.39%). The results suggest a neuroprotective potential response of glia to N. caninum infection under IFN-gamma stimulus. This observation contributes to understand the immune mediated mechanisms of neosporosis in central nervous system (CNS). |
Palavras-chave: | Neospora caninum neuron/glial co-culture immune response parasite NO downmodulation neurite impairment |
País: | Brasil |
Editora / Evento / Instituição: | Frontiers in Cellular Neuroscience |
Citação: | de Jesus et al, 2015 |
Tipo de Acesso: | Acesso Aberto |
URI: | http://repositorio.ufba.br/ri/handle/ri/20780 |
Data do documento: | 27-Out-2014 |
Aparece nas coleções: | Artigo Publicado em Periódico (ICS) |
Arquivo | Descrição | Tamanho | Formato | |
---|---|---|---|---|
de Jesus et al 2014.pdf | 893,96 kB | Adobe PDF | Visualizar/Abrir |
Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.