Estudo fitoquímico do extrato em acetato de etila das raízes de kielmeyera reticulata.

Abstract

This study describes the isolation and structural identification of secondary metabolites present on the extract in ethyl acetate obtained from the roots of the Kielmeyera reticulata Mart. (Calophyllaceae) species, collected in the sandbanks of the Parque Metropolitano do Abaeté, Salvador-Bahia. Species in this family are primarily known as sources of xanthones, which are considered their chemosystematics markers. The roots were subjected to extraction with ethyl acetate. The fractionation of the extract was carried out by vacuum flash silica column chromatography, dry packed, with the solid sample inserted, using hexane and dichloromethane in increasing order of polarity as eluent system, and the compounds were isolated by preparative chromatography on silica gel using hexane and ethyl ether as an eluting system. Compound identification was performed using spectroscopic methods such as onedimensional (1H and 13C) and two-dimensional Nuclear Magnetic Resonance (gHMBC and gHSQC). The following compounds were isolated and identified from the extract: three phloroglucinol derivatives, three biphenyls, one 4-alkylcoumarin, one xanthone and the steroids β-sitosterol and stigmasterol. Three new substances were isolated and identified: a prenylated derivative of phloroglucinol and two biphenyls.