https://repositorio.ufba.br/handle/ri/5063
Tipo: | Artigo de Periódico |
Título: | Schistosoma mansoni antigen-driven interleukin-10 production in infected asthmatic individuals |
Título(s) alternativo(s): | Memórias do Instituto Oswaldo Cruz |
Autor(es): | Oliveira, Sergio Costa Pacífico, Lucila Grossi Gonçalves Goes, Alfredo Miranda de Oliveira, Ricardo Riccio Cardoso, Luciana Santos Araujo, Maria Ilma Andrade Santos Fonseca, Cristina Toscano Carvalho Filho, Edgar Marcelino de |
Autor(es): | Oliveira, Sergio Costa Pacífico, Lucila Grossi Gonçalves Goes, Alfredo Miranda de Oliveira, Ricardo Riccio Cardoso, Luciana Santos Araujo, Maria Ilma Andrade Santos Fonseca, Cristina Toscano Carvalho Filho, Edgar Marcelino de |
Abstract: | Asthmatics infected with Schistosoma mansoni have a less severe course of asthma and an inhibition of the Th2 inflammatory response that seems to be mediated by interleukin (IL-10). The objective of this study was to evaluate the capacity of some S. mansoni antigens to stimulate IL-10 production in vitro by cells of asthmatic infected individuals. Peripheral bloods mononuclear cells were stimulated with the S. mansoni recombinant antigens Sm22.6, Sm14, P24, and PIII antigen. IL-10 was measured in the supernatants of cultures. As the recombinant antigens were cloned in Escherichia coli, we blocked contaminant endotoxin with polymyxin B added to the cultures. We demonstrated that all antigens used drove high production of IL-10 in S. mansoni infected individuals (n = 13, 408 ± 514 and 401 ± 383 pg/ml, 484 ± 245 pg/ml, 579 ± 468 pg/ml, respectively). In asthmatics infected with S. mansoni (n = 21) rP24 induced higher levels of IL-10 (565 ± 377 pg/ml) when compared to PIII, rSm14 and rSm22.6 (184 ± 209 pg/ml; 292 ± 243 pg/ml; 156 ± 247 pg/ml, respectively). Conclusion: the S. mansoni antigens evaluated in this study stimulated IL-10 production by cells from infected individuals and therefore they have the potential to be used as a modulator of the inflammatory response in asthma. |
Palavras-chave: | Schistosoma mansoni recombinant proteins - S mansoni antigens - interleukin-10 |
URI: | http://www.repositorio.ufba.br/ri/handle/ri/5063 |
Data do documento: | Out-2006 |
Aparece nas coleções: | Artigo Publicado em Periódico (Faculdade de Medicina) |
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Schistosoma mansoni antigen-driven.pdf | 212,66 kB | Adobe PDF | Visualizar/Abrir |
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