Skip navigation
Universidade Federal da Bahia |
Repositório Institucional da UFBA
Use este identificador para citar ou linkar para este item: https://repositorio.ufba.br/handle/ri/7048
Tipo: Artigo de Periódico
Título: Detection of specific IgE antibodies in parasite diseases
Título(s) alternativo(s): Brazilian Journal of Medical and Biological Research
Autor(es): Atta, Maria Luiza Brito de Sousa
Araujo, Maria Ilma Andrade Santos
D'Oliveira Junior, Argemiro
Jesus, A. Ribeiro de
Almeida, Roque Pacheco de
Atta, Ajax Mercês
Carvalho Filho, Edgar Marcelino de
Autor(es): Atta, Maria Luiza Brito de Sousa
Araujo, Maria Ilma Andrade Santos
D'Oliveira Junior, Argemiro
Jesus, A. Ribeiro de
Almeida, Roque Pacheco de
Atta, Ajax Mercês
Carvalho Filho, Edgar Marcelino de
Abstract: Activation of Th1 or Th2 cells is associated with production of specific immunoglobulin isotypes, offering the opportunity to use antibody measurement for evaluation of T cell function. Schistosomiasis and visceral leishmaniasis are diseases associated with Th2 activation. However, an IgE response is not always detected in these patients. In the present study we evaluated specific IgE antibodies to S. mansoni and L. chagasi antigens by ELISA after depletion of serum IgG with protein G immobilized on Sepharose beads or RF-absorbent (purified sheep IgG antibodies anti-human IgG). In schistosomiasis patients, specific IgE to SWAP antigen was demonstrable in only 10 of 21 patients (48%) (mean absorbance ± SD = 0.102 ± 0.195) when unabsorbed serum was used. Depletion of IgG with protein G increased the number of specific IgE-positive tests to 13 (62%) and the use of RF-absorbent increased the number of positive results to 20 (95%) (mean absorbances ± SD = 0.303 ± 0.455 and 0.374 ± 0.477, respectively). Specific IgE anti-L. chagasi antibodies were not detected in unabsorbed serum from visceral leishmaniasis patients. When IgG was depleted with protein G, IgE antibodies were detected in only 3 (11%) of 27 patients, and the use of RF-absorbent permitted the detection of this isotype in all 27 visceral leishmaniasis sera tested (mean absorbance ± SD = 0.104 ± 0.03). These data show that the presence of IgG antibodies may prevent the detection of a specific IgE response in these parasite diseases. RF-absorbent, a reagent that blocks IgG-binding sites and also removes rheumatoid factor, was more efficient than protein G for the demonstration of specific IgE antibodies.
Palavras-chave: IgE antibodies
immunoassays
schistosomiasis mansoni
visceral leishmaniasis
Kala-azar
Editora / Evento / Instituição: Brazilian Journal of Medical and Biological Research
URI: http://www.repositorio.ufba.br/ri/handle/ri/7048
Data do documento: 1999
Aparece nas coleções:Artigo Publicado em Periódico (FAR)

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
Souza-Atta, M.L.B..pdf165,98 kBAdobe PDFVisualizar/Abrir
Mostrar registro completo do item Visualizar estatísticas


Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.