https://repositorio.ufba.br/handle/ri/7598
Tipo: | Artigo de Periódico |
Título: | IgG anti-Ige autoantibodies in visceral leishmaniasis |
Título(s) alternativo(s): | Memórias do Instituto Oswaldo Cruz |
Autor(es): | Atta, Ajax Mercês Atta, Maria Luiza Brito de Sousa D'Oliveira Junior, Argemiro Almeida, Roque Pacheco de Araujo, Maria Ilma Andrade Santos Carvalho Filho, Edgar Marcelino de |
Autor(es): | Atta, Ajax Mercês Atta, Maria Luiza Brito de Sousa D'Oliveira Junior, Argemiro Almeida, Roque Pacheco de Araujo, Maria Ilma Andrade Santos Carvalho Filho, Edgar Marcelino de |
Abstract: | Procedures for IgG depletion in visceral leishmaniasis (VL) and schistosomiasis sera using Sepharose-protein G beads also deplete IgE. In this study, the presence of IgG anti-IgE autoantibodies in sera from patients with VL (n = 10), and hepatic-intestinal schistosomiasis (n = 10) and from healthy individuals (n = 10) was investigated. A sandwich ELISA using goat IgG anti-human IgE to capture serum IgE and goat anti-human IgG peroxidase conjugate to demonstrate the binding of IgG to the IgE captured was performed. VL sera had higher titers (p < 0.05) of IgG anti-IgE autoantibodies (OD = 2.01 ± 0.43) than sera from healthy individuals (OD = 1.35 ± 0.16) or persons infected with Schistosoma mansoni (OD = 1.34 ± 0.18). The immunoblotting carried out with eluates from Sepharose-protein G beads used to deplete IgG from these sera and goat anti-human IgE peroxidase conjugate, showed a similar pattern of bands, predominating the 75 kDa e-heavy chain and also polypeptides resulting from physiological enzymatic digestion of IgE. A frequent additional band immediately above 75 kDa was observed only in VL sera. |
Palavras-chave: | IgE IgG anti-IgE protein G visceral leishmaniasis schistosomiasis mansoni |
URI: | http://www.repositorio.ufba.br/ri/handle/ri/7598 |
Data do documento: | Jan-2002 |
Aparece nas coleções: | Artigo Publicado em Periódico (FAR) |
Arquivo | Descrição | Tamanho | Formato | |
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4291.pdf | 72,97 kB | Adobe PDF | Visualizar/Abrir |
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