https://repositorio.ufba.br/handle/ri/14748
Tipo: | Artigo de Periódico |
Título: | Antiproliferative, proapoptotic and morphogenic effects of the flavonoid rutin on human glioblastoma cells |
Título(s) alternativo(s): | Food Chemistry |
Autor(es): | Santos, B. L. Silva, A. R. Pitanga, B. P. S. Sousa, C. S. Grangeiro, M. S. Fragomeni, B. O. Coelho, P. L. C. Oliveira, M. N. Menezes Filho, N. J. Costa, Maria de Fátima Dias El-Bachá, Ramon dos Santos Velozo, Eudes da Silva Sampaio, G. P. Freire, S. M. Tardy, Marcienne Bloch Costa, Silvia Lima |
Autor(es): | Santos, B. L. Silva, A. R. Pitanga, B. P. S. Sousa, C. S. Grangeiro, M. S. Fragomeni, B. O. Coelho, P. L. C. Oliveira, M. N. Menezes Filho, N. J. Costa, Maria de Fátima Dias El-Bachá, Ramon dos Santos Velozo, Eudes da Silva Sampaio, G. P. Freire, S. M. Tardy, Marcienne Bloch Costa, Silvia Lima |
Abstract: | In this study, we investigated the effects of the flavonoid rutin (3,3′,4′,5,7-pentahydroxyflavone-3-rutinoside) on glioma cells, using the highly proliferative human cell line GL-15 as a model. We observed that rutin (50–100 μM) reduced proliferation and viability of GL-15 cells, leading to decreased levels of ERK1/2 phosphorylation (P-ERK1/2) and accumulation of cells in the G2 phase of the cell cycle. On the other hand, 87.4% of GL-15 cells exposed to 100 μM rutin entered apoptosis, as revealed by flow cytometry after AnnexinV/PI staining. Nuclear condensation and DNA fragmentation were also observed, further confirming that apoptosis had occurred. Moreover, the remaining cells that were treated with 50 μM rutin presented a morphological pattern of astroglial differentiation in culture, characterised by a condensed cell body and thin processes with overexpression of GFAP. Because of its capacity to induce differentiation and apoptosis in cultured human glioblastoma cells, rutin could be considered as a potential candidate for malignant gliomas treatment. |
Palavras-chave: | Rutin Flavonoid Gliomas Apoptosis Differentiation P-ERK GFAP |
Tipo de Acesso: | Acesso Aberto |
URI: | http://repositorio.ufba.br/ri/handle/ri/14748 |
Data do documento: | 2011 |
Aparece nas coleções: | Artigo Publicado em Periódico (ICS) |
Arquivo | Descrição | Tamanho | Formato | |
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B. L. Santos.pdf | 870,06 kB | Adobe PDF | Visualizar/Abrir |
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