Avaliação da TFDa associando a luz laser vermelho (660nm) ao composto fenotiazinico azul de taylor para o tratamento de canais radiculares infectados com enterococcus faecalis

Abstract

Enterococcus faecalis is the main pathogen responsible for failures in endodontic treatments in clinical dentistry. Its ability to penetrate dentinal canaliculi, resist to large variations in pH, high capacity to produce biofilm, resistance genes and efflux pumps makes it highly resistant to conventional pharmacotherapies and classical endodontic treatments. The aim of this study was to evaluate the efficacy of in vitro photodynamic therapy using Taylor blue in nanocontractions of 332 ηg/mL and 689.9 ηg/mL associated with red laser coupled to optical fiber (λ660 ηm) in pre-irradiation times of 5 and 10 minutes on the entire biofilm of E. faecalis in root canals. All experiments were performed in triplicate and the results used the ANOVA statistical test with Tukey's multiple comparison and post-test the GraphPad Prism® 6.0 software. TFDa was performed in two protocols 1 and 2, which showed significant reductions in microbial load (p<0.001 and p<0.01) when compared to the positive control group. The percentage of microbial reduction of intracanal E. faecalis whole biofilm culture in these groups were 90% in protocol 1 and 90.3% in protocol 2. Conclusion, the findings demonstrated that TFDa showed promise when used against E. faecalis whole biofilm in the root canal, making it a viable, therapeutic alternative for use in dentistry because it is efficient, can be modulated according to the need for treatment, and there is no evidence of induction of resistant strains of the microorganism.